EFB Dengue Literature Review

CD10

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CD10

Overview

CD10 (neutral endopeptidase; neprilysin) is expressed on B cell precursors, transitional B cells, and germinal centre B cells in humans. Its expression marks immature or GC-associated B cells; absence of CD10 in combination with IgD⁻CD27⁻ phenotype is used to confirm that DN B cells are not transitional or pre-GC contaminants.

Key Points from Literature

  • DN B cells are CD10⁻ in peripheral blood, distinguishing them from transitional B cells (CD10⁺ CD38^high IgD⁺), pre-GC cells (CD10⁺ CD38^high IgD⁺/⁻), and GC cells in tonsil (CD10⁺ CD38^high) (see Wei2007 - DN Memory B Cells in SLE, 8-color multiparameter analysis).

  • In tonsil, the DN counterpart (IgD⁻CD27⁻CD10⁻ Bm5 fraction) is distinguished from tonsillar GC cells (IgD⁻CD27⁻CD10⁺ CD38^high) by CD10 and CD38 expression (see Wei2007 - DN Memory B Cells in SLE).

  • A small fraction of isotype-switched CD27⁺ and DN cells in PBL express CD10, likely representing recirculating pre-GC cells (see Wei2007 - DN Memory B Cells in SLE).

  • CD10⁻ in acN cells: The absence of CD10 expression on activated naive (acN) cells (IgD⁺CD27⁻MTG⁺CD24⁻CD21⁻) was used to argue against misclassification of these cells as late transitional B cells, which can share IgD⁺MTG⁺ phenotype with acN cells but are CD10⁺. CD10⁻ status thus helps confirm the acN cell identity alongside CD24⁻ and CD38^lo (see Tipton2015 - ASC Diversity and Origin in SLE).

  • CD10⁻ gate for mature B cells in dengue: GarciaBates2013 gated on CD19⁺CD10⁻ cells to define the mature B cell population before further subset analysis by CD27/CD21/CD20/CD38. This excludes transitional and GC-derived B cells from the analysis and is a standard approach for dengue B cell phenotyping (see GarciaBates2013 - Plasmablast Response and Dengue Severity, LSRII FCM, n=84 dengue).

  • CD10 is named as one of the exclusion markers that keeps transitional cells out of a CD21ˡᵒ activation gate. The review warns that early transitional (T1) B cells are themselves CD21ˡᵒ and must be excluded using CD38, CD24 and/or CD10 — without that exclusion, a CD21-anchored DN2 or activated-memory gate can be contaminated from below by developmentally immature cells. Table 1 gives T1/T2 as CD10⁺⁺/⁺ (see Sanz2019 - Consistent Classification of Human B Cell Populations, review — no original data). See DN2 Gating Strategy.

Contradictions & Debates

None documented in current wiki sources.

Double-Negative B Cell, Activated Naive B Cell, CD38, Germinal Center, Conventional Flow Cytometry

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