EFB Dengue Literature Review

B220

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B220

Overview

B220 (CD45R; the RA isoform of CD45) is expressed on B lineage cells and is the equivalent of mouse B220. In humans, B220 expression is heterogeneous among B cell subsets: naive B cells universally express B220, while memory B cells can be divided into B220⁺ and B220⁻ fractions. Plasma cells downregulate B220.

Key Points from Literature

  • Conventional CD27⁺ memory cells can be divided into B220⁺ and B220⁻ subsets; this heterogeneity is also found in DN B cells, confirming their similarity to conventional memory cells at this marker (see Wei2007 - DN Memory B Cells in SLE).

  • Naive B cells universally express B220; memory cells (both CD27⁺ and DN) show partial downregulation (see Wei2007 - DN Memory B Cells in SLE, citing Bleesing & Fleisher 2003).

  • B220 anchors both founding murine ABC definitions. Hao et al. 2011 and Rubtsov et al. 2011 both gated ABCs as B220⁺CD19⁺ splenocytes before applying their respective (and non-identical) additional marker criteria (see Cancro2020 - Age-Associated B Cells, review — no original data; mouse).

  • B220 carries two distinct roles in Song2022 — flow gate and in-situ landmark. Every population in the study is defined inside a B220⁺ gate: naive follicular B220⁺CD19⁺IgD^hi^CD23⁺, GC B220⁺IgD^lo^CD95⁺GL-7⁺, and T-bet⁺CD11c⁺ B220⁺CD19⁺CD44^hi^CD11c⁺T-bet⁺. In confocal histocytometry, anti-B220 together with PNA labels the germinal centre and follicle in tissue, and it is on that basis that T-bet⁺CD11c⁺ B cells were found “largely absent from PNA- and B220-labeled GCs” and localised instead to the follicular mantle at the outer edge of the follicle (see Song2022 - Tfh Outside Germinal Centers Drive T-bet CD11c B Cells, mouse, LCMV-Armstrong + influenza PR8, n=3–5 mice/group). Tissue B cells were identified as CD4⁻B220⁺ in the histocytometry gating.

Contradictions & Debates

None documented in current wiki sources.

Double-Negative B Cell, Memory B Cell, CD27

Sources

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